Quantification and statistical analysis

Quantitative analyses were carried out on at least 3 independent animals of each genotype. All statistical analyses and the generation of graphs were performed using the GraphPad software.

Statistical tests used are specified in the legends of the figures and supplementary figures wherever appropriate. In cases in which our datasets did not follow normal distribution, nonparametric statistical tests have been applied. In cases in which our datasets do not contain enough biological replicates to be able to determine whether they follow a normal distribution or not, non-parametric tests have been also applied. No statistical methods have been used to determine sample sizes.