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2.7. Transmission electron microscopy (TEM)
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Under anaesthesia, the fresh SN of mice were harvested and fixed with 2.5% glutaraldehyde, post‐fixed with 1% OsO4 in 1% PB (pH 7.4), dehydrated in a graded series of ethanol, and embedded in resin, cut to 60–80 nm ultrathin section and finally stained by standard procedures. Autophagosomes were observed under a HT7800 transmission electron microscope (Hitachi).
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