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Slice preparation for electrophysiology and calcium imaging experiments

KT Kathryn R. Taylor
TB Tara Barron
AH Alexa Hui
AS Avishay Spitzer
BY Belgin Yalçin
AI Alexis E. Ivec
AG Anna C. Geraghty
GH Griffin G. Hartmann
MA Marlene Arzt
SG Shawn M. Gillespie
YK Yoon Seok Kim
SJ Samin Maleki Jahan
HZ Helena Zhang
KS Kiarash Shamardani
MS Minhui Su
LN Lijun Ni
PD Peter P. Du
PW Pamelyn J. Woo
AS Arianna Silva-Torres
HV Humsa S. Venkatesh
RM Rebecca Mancusi
AP Anitha Ponnuswami
SM Sara Mulinyawe
MK Michael B. Keough
IC Isabelle Chau
RA Razina Aziz-Bose
IT Itay Tirosh
MS Mario L. Suvà
MM Michelle Monje
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Coronal slices (300 µm thick) containing the hippocampal region were prepared from mice (4–8 weeks after xenografting) in accordance with a protocol approved by Stanford University Institutional Animal Care and Use Committee (IACUC). After rapid decapitation, the brain was removed from the skull and immersed in ice‐cold slicing artificial cerebrospinal fluid (ACSF) containing (in mM): 125 NaCl, 2.5 KCl, 25 glucose, 25 NaHCO3 and 1.25 NaH2PO4, 3 MgCl2 and 0.1 CaCl2. After cutting, slices were incubated for 30 min in warm (30 °C) oxygenated (95% O2, 5% CO2) recovery ACSF containing (in mM): 100 NaCl, 2.5 KCl, 25 glucose, 25 NaHCO3, 1.25 NaH2PO4, 30 sucrose, 2 MgCl2 and 1 CaCl2 before being allowed to equilibrate at room temperature for an additional 30 min.

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