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Stained tissue sections were imaged on an Olympus BX 51 microscope or an Olympus FV3000 confocal microscope system. Tiled fields were stitched using a 15% overlap. Images were processed by Adobe Photoshop CC software.
ImageJ software was applied to evaluate the expression level, determined by average optical density (EGFR, Bmp4, and Notch3) and the overall population of myelin, defined by integrated density (MBP).
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