Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Oxygen consumption rate

XS Xingzong Sun
MQ Menghan Qian
HL Hongliang Li
LW Lei Wang
YZ Yunjie Zhao
MY Min Yin
LD Lili Dai
HB Hongkun Bao
ask Ask a question
Favorite

Cells were pre-inoculated in transparent bottom black 96-well plates and treated according to the pre-designed cells. On the day of the test, two columns (3–6 groups in parallel) were taken to add Carbonyl cyanide-p-trifluoromethoxyphenylhydrazone (FCCP) and Rotone as the control, and three columns were selected as the blank control. The pure medium, mixed isobaric (Dojindo, E297) dye, and the cells of the untreated group were added. The cells of the other experimental groups were replaced by the mixed isobaric dye medium immediately after blocking the air with a drop of mineral oil in each well, and the data were collected by the fluorescent zymography at the wavelengths of 500–650 nm at intervals of 10 min and statistically analyzed.

Copyright and License information: The Author(s) ©2023
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A