2.6. Tumor immune infiltration, immune checkpoint molecules, and immune cell pathways

Based on the the tumor immunity estimation resource database, the relationship between HDAC7 expression in DLBCL and 6 types of immune-infiltrating cells, including B cells, CD4⁺ T cells, CD8⁺ T cells, neutrophils, macrophages, and dendritic cells, was analyzed. Tumor immune system interactions (http://cis.hku.hk/TISIDB/) was used to explore HDAC7 expression in tumor tissues and tumor-infiltrating lymphocytes (TILs).^[13] After analyzing the gene expression profiles, gene set variation analysis was used to determine the relative abundance of the TILs. We also calculated immune cell infiltration in DLBCL using CIBERSORT with P < .001 as the cutoff value.^[14] We conducted these analyses using the “limma” R package and visualized this using the “reshape2” and “RColorBrewer” R packages. The aforementioned analyses were performed using the Sangerbox tools (http://www.sangerbox.com/tool).