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Co-transfer of Ly49H+ NK cells and MCMV infection

TN Tsukasa Nabekura
ED Elfira Amalia Deborah
ST Saeko Tahara
YA Yuya Arai
PL Paul E. Love
KK Koichiro Kako
AF Akiyoshi Fukamizu
MM Masafumi Muratani
AS Akira Shibuya
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Tyrobp–/– mice received 1 × 105 CD45.1+ WT Ly49H+ NK cells and either 1 × 105 CD45.2+ Themis2–/– or Zfp740–/– Ly49H+ NK cells and then infected with MCMV, as described previously22.

To analyze the secondary expansion of memory NK cells, Tyrobp–/– mice received 1.5 ×106 CD45.1+ WT Ly49H+ NK cells and 1.0 × 106 CD45.2+ Themis2–/– Ly49H+ NK cells and then infected with MCMV. On day 28 pi, donor memory CD45.1+ WT and CD45.2+ Themis2-/- NK cells were enriched from the spleen of the recipient mice as described above. Naïve Tyrobp–/– mice received 3.0 × 105 memory CD45.1+ WT and CD45.2+ Themis2–/– NK cells and then infected with MCMV.

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