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4.10. Bioinformatics Methods

PS Pingyu Sun
GZ Guoyu Zhang
MX Ming Xian
GZ Guangzhi Zhang
FW Fei Wen
ZH Zhangtao Hu
JH Jianhong Hu
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The eggNOG online database http://eggnog5.embl.de/#/app/home (accessed on 30 June 2023) and KEGG-mapper online tool http://www.kegg.jp/kegg/mapper.html (accessed on 30 June 2023) were utilized to perform Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses of the DEPs identified between HF and LF spermatozoa. Pathways with a corrected p-value < 0.05 were considered significantly enriched. Protein–protein interactions (PPIs) were investigated by searching all DEPs database accession or sequence against the STRING (version 11.5) database https://cn.string-db.org/ (accessed on 30 June 2023).

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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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