Mouse breast cancer cell line 4T1 and mouse macrophage RAW264.7 (M0 macrophage) were purchased from the Cell Bank of Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences (Shanghai, China) and cultured in DMEM supplemented with 1% penicillin/streptomycin and 10% fetal bovine serum (FBS, v/v) in a humidified incubator with 5% CO2 at 37 ℃.
Female Balb/c mice (6 ~ 8 weeks) were purchased from the Shanghai Silaike Laboratory Animal Limited Liability Company. The experiments were performed in accordance with national regulations and was approved by Ethics Committee of the Institution on Laboratory Animal Care in Zhejiang University.
Copyright and License information: The Author(s) ©2023 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this
article to respond.
Ask a
question
Post a Question
