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HaCaT cell monolayers in 6 wells in triplicates were washed with PBS and infected with MOI 10 of HSV-1 Syn 17 + . The virions in 1 mL of DMEM P/S were adsorbed at 37 °C with periodical rocking of the plates for 1 h, inoculum removed and cells washed with PBS, followed by addition of 2 mL/well of DMEM supplemented with 10% FCS and P/S. Virus was harvested at 17 h post infection. Media was spun at 3000 rpm for 10 min at 4 °C, supernatant aliquoted and stored at −80 °C. Cell monolayers were washed with PBS, dissociated with TrypLE reagent, spun at 100 × g for 5 min, washed with PBS, aliquoted and spun at 400 × g for 5 min, supernatant removed and cells stored at −80 °C.
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