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2.11. TdT‐mediated dUTP nick end labelling assay and Ki67 immunostaining
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After decalcification, DNA strand breaks were detected using a TdT‐mediated dUTP nick end labelling staining kit (Roche, Nutley, NJ). Cell proliferation was detected using the Ki67 antibody (Abcam, Cambridge, UK), and the nuclei were stained with 4′,6‐diamidino‐2‐phenylindole. Images were captured using the IX 70 fluorescence microscope (Olympus).
Copyright and License information: ©2023 The Authors. published by Beijing Institute for Stem Cell and Regenerative Medicine and John Wiley & Sons Ltd.
This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
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