Human liver specimens

Formalin fixed and Paraffin embedded (FFPE) human liver tissues were obtained from the First Laboratory of Pathology, Medical School, NKUA, Greece, or Clinical Pathology and Pathology Division, Karolinska Institute, Sweden. All human samples were used according to declaration of Helsinki and under an Institutional Review Board–approved protocol and with support from the Liver Tissue Procurement and Distribution System (HHSN 267200700004CN01-DK-7-0004). A policy of strict anonymity and confidentiality was assured according to Ethics Committee. Biopsies from 2 available post-mortem AHF patients (males), as well as, a case with nearly normal liver histology (male) serving as negative control, were analyzed for the validation of MFGE-8 expression.