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Mice were prepared for surgery as described above. The hair on the neck was removed and disinfected. A mid-line incision (about 1.5–2 cm) was made, and nodose ganglia on both sides were carefully exposed. A volume of 400 nl AAV.PHP.S-DIO-gCOMET or 300 nl AAV.PHP.S-mCherry-Flex-DTA/AAV2-hSyn-DIO-mCherry was injected into the nodose bilaterally at a speed of 150 nl min–1. Mice were euthanized 3–6 weeks after surgery for tissue collection.
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