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Lifespan analysis was conducted following our previous protocol.38 Briefly, worms were cultured on NGM plates and seeded with E. coli OP50. Fluorodeoxyuridine (FUDR, 50 μM) was used to prevent the generation of F2 animals. Worms were transferred to new FUDR plates on Day 3 and Day 6 by washing. The survival rates were scored every day, with the first day of adulthood set as Day 1. A worm was considered dead when it did not respond to touch. The survival plot and statistical analysis were completed with online OASIS2 resources.60
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