Incucyte live-cell analyses

IR Ilaria Rosso
CJ Corey Jones-Weinert
FR Francesca Rossiello
MC Matteo Cabrini
SB Silvia Brambillasca
LM Leonel Munoz-Sagredo
ZL Zeno Lavagnino
EM Emanuele Martini
ET Enzo Tedone
MG Massimiliano Garre’
JA Julio Aguado
DP Dario Parazzoli
MM Marina Mione
JS Jerry W. Shay
CM Ciro Mercurio
FF Fabrizio d’Adda di Fagagna
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Cells plated in 96 MW and treated as indicated were monitored overtime using Incucyte S3 or S5 live-cell analysis system (Sartorius). For detection of apoptotic cells, Incucyte caspase 3/7 green dye for apoptosis (Sartorius) was added 1:1000 to cells one day after ASO transfection, to prevent interaction between ASO and dye. Growth rate (GR) was calculated as reported in ref. 63. For IC50 calculation, values were computed using Prism software.

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