Quantification of RT-qPCR data

BK Basma M. Klump
GP Gloria I. Perez
EP Eric M. Patrick
KA Kate Adams-Boone
SC Scott B. Cohen
LH Li Han
KY Kefei Yu
JS Jens C. Schmidt
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RT-qPCR experiments were carried out in triplicate and the TR Ct value was normalized to the GAPDG Ct value. The mean ΔCt (Ct of TR – Ct of GAPDH) value from three independent experiments and the corresponding standard deviation were plotted.

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