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Mouse neonatal cardiomyocyte (MNC) isolation
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Cardiac cells were isolated from day-old neonatal mouse hearts, previously segregated by sex49, in sterile conditions as described in the mouse and rat neonatal heart dissociation kit protocol (Miltenyi Biotec 130-098-373). Pipetting approximately 1.6 × 105 cells per well of glass bottom Ibidi plates covered with a layer of 1% gelatin. Cells were maintained at 37 °C with 5% of CO2.
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