Tissues were fixed for 2 h in 2.5% glutaraldehyde/2% paraformaldehyde in 0.1 M Na cacodylate buffer, washed in 0.1 M Na cacodylate buffer, fixed for 1-h in 1% osmium/1.5% K-ferricyanide in 0.1 M Na cacodylate buffer, dehydrated in ethanol, and finally embedded in EPON (all were obtained from Electron Microscopy Sciences, USA). Thin sections were viewed using Tecnai-12 transmission electron microscope and photographed by CCD camera MegaView II and software AnalySIS 3.0.
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