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Scaffold and tumor samples were fixed in 3% buffered formalin for 1 h and immersed in increasing concentrations of alcohol to dehydrate the tissues prior to paraffin embedding. Xylene and Ultraclear (J.T. Baker) were used as clearing and deparaffinizing agents for tumor samples and scaffolds respectively. Sections were stained using a standard H&E and immunohistochemistry protocol applied in clinical pathology. For antibody list see Table S7. Images were quantified with Image J.
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