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Total RNA was extracted from E11.5 WT and CKO limb bud cells and the libraries were constructed using the VAHTS Stranded mRNA-seq Library Prep Kit for Illumina v2. The libraries’ quality was checked using Qubit 2100, and the concentrations were determined from the analysis profiles. Genes expression with a change fold ≥2 or ≤ −2, p value < 0.05 were identified as differentially expressed genes, and the GO enrichment analysis and KEGG pathway analysis were performed using DAVID [37].
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