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Kidney tissue sections were dewaxed to water and stained with hematoxylin for a duration of 20 min. Subsequently, the sections were placed in warm water at 50 °C until a blue color appeared. Afterward, the sections were stained with eosin for 5 min, and sealed with neutral gum. Finally, the sections were observed using an electron microscope (Motic China Group Co., Ltd, China) at magnifications of 100× and 400×.
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