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The KCNAB2-expressing lentiviral vector (“LV-KCNAB2”) and the negative control lentiviral vector were provided by Genechem (Shanghai, China). The wells of the 6-well plate were seeded with NSCLC cells using 1 × 105 cells per well. When the cell fusion rate reached 60% in each well, the lentivirus (MOI = 10) was added to cell culture medium. Stable KCNAB2-overexpressed cells were established with puromycin (3 µg/ml). KCNAB2 expression among stable cells was finally determined via qRT-PCR and Western blotting assays.
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