Novel object recognition task (NORT)

The NORT was used to measure hippocampus-dependent short-term memory retention and consisted of a training and a test session. Briefly, mice were allowed to explore the open field box for 30 min per day for habituation prior to testing. Mice were allowed to explore two identical objects (object 1 and 2) for 10 min during the training period. Mice were rested for 6 h, then the test session was conducted in which object 2 was replaced with a novel object of different surface texture, size, and shape. Mice were again housed and allowed to explore freely for 10 min. Mouse behavior was recorded using a digital automatic tracking system (Shanghai Xinruan, China).