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HEK293T cells (ATCC, USA) were cultured in DMEM (10% FBS, 1% penicillin-streptomycin) at a humidified incubator (37 °C, 5% CO2). Transfections of the indicated plasmids carried out at 80-90% confluent cells using Lipofectamine 2000 (Invitrogen). All cells were tested for mycoplasma contamination and confirmed to be mycoplasma-free.
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