All data were analyzed using GraphPad Prism 7 (GraphPad Software Inc., La Jolla, CA, USA) and expressed as mean ± SEM. A comparison of the means among groups was performed using Mann-Whitney U test for body weight, tumor weight, and tumor volume data, or one-way analysis of variance (ANOVA) followed by Dunnett’s post-hoc test for IHC data. Fisher’s exact test, Student’s t test, and Kaplan–Meier survival analysis were used to analyze the relationship between nuclear ATF6 expression and clinicopathological parameters (SPSS software, SPSS, Inc., Chicago, IL, USA). Statistical significance was set at P < 0.05.
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