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Ten-centimeter dishes were used for cultivating 5-8F or S26 cells, and then, they were treated with 10 μM HAMNO for 24 h. After being scraped gently with a cell scraper (Corning, USA), the samples were fixed in 2.5% glutaraldehyde (Sigma‒Aldrich, USA) for 5 min at room temperature after drug treatment. Following overnight fixation in 2.5% glutaraldehyde at 4 °C, the cells were centrifuged at a slower speed (3000 rpm min−1) to collect the precipitate. Electron microscopy (JEM-1400flash) was used to examine the samples at Sun Yat-sen University.
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