Quantification of NETs DNA

TL Tsai-Ling Liao
HL Hung-Jen Liu
DC Der-Yuan Chen
KT Kuo-Tung Tang
YC Yi-Ming Chen
PL Po-Yu Liu
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Briefly, 100 μl of plasma or neutrophil culture supernatant was incubated overnight in a 96-well plate precoated with anti-MPO antibody (Santa Cruz, USA). The DNA bound to MPO was quantified using the Quant-iT PicoGreen kit (Thermo Fisher Scientific, USA) according to the manufacturer’s instructions.

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