2.10. Periodic acid‐schiff (PAS) staining

JH Jijia Hu
ZZ Zongwei Zhang
HH Hongtu Hu
KY Keju Yang
ZZ Zijing Zhu
QY Qian Yang
WL Wei Liang
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PAS staining was performed with Periodic Acid Schiff (PAS) Stain Kit (Servicebio, China), according to the manufacturer's instructions. Slides were examined by light microscope (Olympus, Japan). Mesangial matrix expansion was defined as the increased amounts of PAS‐positive material in the mesangial region and evaluated by the glomerular sclerosis index. 17 Twenty glomeruli per mouse were assessed.

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