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Quantification of RGCs in retinal wholemounts
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Retinal wholemounts were divided into four quadrants. In each quadrant, 4 non-overlapping pictures were taken using a fluorescent microscope (400 x; Axio Observer.D1, Zeiss), proceeding from the center to the periphery. The number of βIII-tubulin positive RGCs was determined and normalized to an area of 1 mm2. Values were averaged per retina and across all animals. Each experimental group included 5–9 retinae. Eyes with lens injury were excluded from the experiment.
Copyright and License information: ©2023, Leibinger, Zeitler et al
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