Stained sections were scanned using the 3DHistech Panoramic 250 Flash II to generate brightfield images. Fluorescent images were captured either on a Olympus IX53 and Nikon Eclipse Ti inverted microscope or the Leica TCS SP8X/MP confocal microscope. Images of whole bone were achieved by stitching together 20–24 images at 10x magnification using ImageJ software.
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