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Annotation of mossy fiber terminals and Purkinje cells
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We manually annotated individual mossy fiber terminals or Purkinje cells in the raw coronal section images to obtain their [x, y] coordinates within each coronal section. We then aligned the coronal sections to the CCF, thereby obtaining their [x, y, z] CCF coordinates. We used the Allen Reference Atlas ontology to assign annotations to neuroanatomical regions based on their CCF coordinates. For plotting three-dimensional brain structures, we used the Allen Reference Brain (ARAv3) meshes.
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