The open field test was carried out as previously described151. In brief, mice were habituated in the behavior room in the presence of background white noise for at least 30 min before the behavior test. Individual mice were then placed in an open field arena (35 cm × 35 cm) by a blinded experimenter and allowed to freely explore the box for 20 min. The total distance, velocity and time spent in the center (center of the arena, 22 cm × 22 cm) were quantified using video tracking software (TopScan, CleverSys). All mice were handled gently to avoid stress in the experiment. Male mice were used in this behavior test.
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