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Intracellular reactive oxygen species (ROS) assay
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After the preparation of the cell samples, a fluorescent probe DHE (38483-26-0, KeyGEN BioTECH) was used for ROS determination. For this process, 10 µM DHE was added to the cells, which were then incubated at 37 ℃ for 20 min in the dark and washed with PBS. Finally, fluorescence images were taken with a LEICA DMi8 fluorescent inverted microscope.
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