4.4. DropViz expression comparison

Cell cluster-aggregated expression levels for NRF2 were obtained from DropViz (http://dropviz.org) [44] for mouse brain single cell RNA-seq using the following steps: 1) on the ‘Query’ tab, Nfe2L2 was entered as Gene and ‘Update!’ was selected, 2) the ‘Global Clusters’ tab was opened as a ‘Table’, and 3) the ‘Download as CSV’ button was used to download a comma-separated table (CSV file) of expression values for Nfe2l2 by region and cell type/cluster. The expression values downloaded from DropViz and plotted here are the mean single-cell UMI gene counts per 100,000 cell type UMI counts, log₁₀-scaled. Due to the relatively smaller number of cell types plotted (compared to the other single cell datasets), these data were plotted in Python (version 3.9.13) using dot plots, geom_dotplot() function, rather than sina plots but were otherwise plotted using the same methodology as the other expression comparisons.