Ingenuity pathway analysis (IPA)

Comparison datasets, i.e., 1) WT AML LICs versus normal Lin⁻Sca-1⁻c-Kit⁺ cells and 2) Gpr44^−/− AML LICs versus WT AML LICs, generated from differential gene expression analysis were uploaded to QIAGEN IPA application (Version 01-20-04). Core and comparison analyses were conducted according to the manufacturer’s instructions. Canonical pathways were analyzed in core analysis in each datasets by setting the cutoffs: LogFC (Expr Log Ratio) −1.4 to 1.4 and p value <0.05. Comparison analysis was performed by comparing the above core analysis results to identify the effect of GPR44 deletion in the background of AML.