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Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses

YS Yi Sun
HC Huiting Chen
SC Sichuan Chen
XX Xueming Xu
WZ Wenchang Zhang
YL Yuchen Li
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mRNAs that were differentially expressed (fold change (FC) ≥ 2.0 and P ≤ 0.05) were screened for GO and KEGG analyses. GO analysis can be divided into three parts: biological process (BP), cellular component (CC) and molecular function (MF). According to the corresponding ID or sequence annotation, relevant proteins or genes can be matched to their corresponding GO numbers and terms, that is, corresponding functional categories or cellular localization. According to the function of molecular involvement, KEGG pathway analysis was performed by analyzing the degree of target gene enrichment in signaling pathways to identify significantly enriched biological signaling pathways, and these analysis were achieved by DAVID.

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