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TUNEL assay of apoptosis in brain tissue
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The tissues were soaked in 10% (v/v) formalin, embedded in paraffin, and then cut into 5-μm sections with a microtome (Leica RM2145, Germany). The sections were dewaxed by sequential immersion in xylene and gradually increasing concentrations of alcohol. TdT-mediated dUTP Nick-End Labeling (TUNEL) was used for detection. The experimental process was operated according to the requirements of the kit (Roche, USA). The image-pro plus system analyzes the total positive cells number observed under optical microscopy (10 randomly selected fields, 400× magnification).
Copyright and License information: ©2023 Li et al.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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