2.1. Chemicals

Perfluorobutanesulfonic acid (PFBS), perfluorohexanoic acid (PFHxA), [undecafluoro-2-methyl-3-oxahexanoic acid (HFPO-DA)], 6:2 fluorotelomer alcohol (6:2 FTOH), perfluorohexanesulfonic acid (PFHxS), Rifampicin (CAS: 13292–46-1), ethanol (EtOH), and dimethyl sulfoxide (DMSO), methanol (MeOH), acetonitrile (ACN) were obtained from Sigma-Aldrich (St. Louis, MO). More detailed information on the PFAS chemicals is in Table 1. Benzo[a]pyrene (CAS: 50–32-8) was obtained from Thermo Scientific Chemicals (Waltham, MA). The fluorogenic substrates and standards for inhibition experiments were 7-benzyloxy-4-(trifluoromethyl) coumarin (7-BFC); 7-ethoxy-4-(trifluoromethyl)coumarin (7-EFC); 7-methoxy-4-(trifluoromethyl) coumarin (7-MFC); 7-hydroxy-4-(trifluoromethyl)coumarin (HFC); 7-methoxyresorufin; and resorufin sodium salt (Sigma-Aldrich, St. Louis, MO). All chemicals and reagents were of analytical grade. William's E Medium, Glutamax™, hydrocortisone hemisuccinate, and insulin were obtained from Gibco Life Technologies (ThermoFisher Scientific, Waltham, MA). Hydrocortisone hemisuccinate and dimethylsulfoxide (DMSO) were obtained from Sigma-Aldrich (St. Louis, MO).

PFAS chemicals used in this study. All chemicals were of analytical grade (≥97%) (CAS: Chemical Abstracts Service. IUPAC: International Union of Pure and Applied Chemistry. MW: Molecular Weight (g/mol)).