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Paraformaldehyde fixation of HEK293T cells
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Samples were harvested as described above and washed twice in PBS (Lonza) and re-suspended in 4% paraformaldehyde (Sigma-Aldrich) for 20 minutes at room temperature. The samples were then washed twice in PBS (Lonza) and filtered using a 40 um cell strainer (Greiner). The cells were counted on a haemocytometer and loaded at a concentration of 250 cells/μL.
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