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A sample of purified protein (25 µg) was digested using ProtiFi S-Trap micro spin columns according to the manufacturer’s protocol. After protein digestion the sample was dried in a vacuum concentrator and resuspended in 725 µl 5% acetonitrile (ACN) and 0.1% formic acid (FA). Part of the sample was transferred to an HPLC vial and further diluted 1:2 prior to analysis.
Copyright and License information: ©2023, Qi, Acosta Gutierrez et al
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