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Cell culture and transfection

XL Xuemei Lu
LH Liqiang Hu
JM Jiayan Mao
SZ Shufen Zhang
YC Ying Cai
WC Wei Chen
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Four human CRC cell lines, including LoVo, SW480, HCT116, and HT29, and a normal human colon epithelial cell line, NCM460, were purchased from the Procell Life Science & Technology Co., Ltd. All cells were cultured in RPMI 1640 medium or DMEM (Gibco; Thermo Fisher Scientific, Waltham, MA, USA) with 10% fetal bovine serum (Gibco; ThermoFisher) at 37 °C in a 5% CO2 atmosphere.

HT29 and HCT116 cells were transfected with siRNA targeting ANXA9 (si-ANXA9-1, si-ANXA9-2, si-ANXA9-3) or negative control siRNA (Gene Pharma, Suzhou, China) using OPTI-MEM (Invitrogen; Thermofisher) and Lipofectamine 2000 (Invitrogen; ThermoFisher) according to the manufacturer’s instructions. The ANXA9 siRNA sequences used were as follows: si-ANXA9-1:5′-GGCAGCUCAUCUCACGAAATT-3′; si-ANXA9-2: 5′- GGACGUGGCCAUUGAAAUUTT-3′; si-ANXA9-3: 5′- GCAGUCUACAAACACAAUUTT-3′.

Copyright and License information: The Author(s) ©2023
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