Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Concise Method
Cardiac tissue sampling and index detection
Request a Protocol
Ask a
question
Favorite
After the blood oxygen index of the animal was measured, the animal was anesthetized with 1% pentobarbital sodium (50 mg/kg), the chest and abdominal cavity were opened. The heart was removed after the blood was collected, the residual blood was removed and weighed, and the cardiac index (heart weight/body weight × 100%) was calculated. Masson staining was performed for morphologic assessment by routine fixation in 4% paraformaldehyde fluid routine.
Copyright and License information: The Author(s) ©2023
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
