Combinations of the most stable genes (PHOS and EF1α; MNP3 and EF1α) and the least stable reference genes (LAC and VP) were compared for normalization of the relative expression of the CHU_2040 gene, which encodes an esterase enzyme, potentially involved in phorbol ester degradation. RNA extraction, cDNA synthesis and qPCR were all conducted as previously described. The software qbase + (Biogazelle) was employed to calculate the average quantification cycles (Cqs) per gene, with the software GraphPad Prism v7 employed for statistical analysis of the combined condition.
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