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Animal care and use

SS Sami Sbei
TM Taylor Moncrief
NL Nathachit Limjunyawong
YZ Yaping Zeng
DG Dustin P. Green
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All experiments were performed with the protocols approved by the Animal Care and Use Committee of the University of Texas Medical Branch. All mice used were 6 to 12 weeks old. WT littermates were used in behavioral experiments with MrgprB2−/− mice. The mice were housed in the vivarium with a 12-h light/dark cycle and all the behavioral tests were performed from 9 am to 2 pm in their light cycle. The housing group was five mice at maximum. All mice used were in the C57bl/6j mice background. A total of 137 mice were used. 98 mice for in vivo experiments, and 39 mice for in vitro experiments.

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