Media used for isolation and bacterial enrichment

The culture media MMSw by Marine Medium Shewanella, is a modified culture media used to isolate facultative microorganisms contains NH₄Cl 1.49 g/L, KCl 0.099 g/L, NaH₂PO₄ 0.599 g/L, Na₂SO₄ 0.099 g/L, MgSO₄*7H₂O 0.246 g/L, CaCl₂ 0.022 g/L, PIPES 6.04 g/L, Na₂SeO₄ 1 mM and NaCl 21 g/L, it was supplemented with 10 ml/L of two solutions DL Minerals and DL vitamins reported by Coppi et al. (2001). The culture media MARS from Marine water Supplemented, designed by us for enrich microorganism able to growth in sea water with selected supplements, it contains water collected from the Perdido fold area, sterilized by filtration and supplemented with NH₄Cl 0.19 g/L as nitrogen source and K₂HPO₄ 0.006 g/L, KH₂PO₄ 0.012 g/L as phosphate source, 10 ml/L of DL Minerals solution and DL Vitamins solution (Coppi et al. 2001). Both media were supplemented with acetate 20 mM or hydrocarbon as carbon sources.

The composition of the DL trace mineral solution per liter of deionized water is 1.5 g of nitriloacetic acid, 3.0 g of MgSO₄, 0.5 g of MnSO₄*H₂O, 1 g of NaCl, 0.1 g of FeSO₄*7H₂O, 0.1 g of CaCl₂*2H₂O, 0.1 g of CoCl₂*6H₂O, 0.13 g of ZnCl₂, 0.01 g of CuSO₄*5H₂O, 0.01 g of AlK(SO₄)₂*12H₂O, 0.01 g of H₃BO₃, 0.025 g of Na₂MoO₄*2H₂O, 0.024 g of NiCl₂*6H₂O and 0.025 g of Na₂WO₄*2H₂O (Coppi et al. 2001).

The composition of the DL vitamin solution per liter of deionized water is 0.0002 g of biotin, 0.005 g of calcium pantothenate, 0.0001 g of vitamin b12, 0.005 g of para-aminobenzoic acid, 0.005 g of thioctic acid, 0.005 g of nicotinic acid, 0.005 g of thiamine hydrochloride, 0.005 g of riboflavin, 0.01 g of pyridoxine hydrochloride and 0.002 g folic acid (Coppi et al. 2001).