Combined extraction (MEF + HHP and HHP + MEF)

A combination of technologies was performed in sequential mode and using the two possible sequences of processes (MEF followed by HHP and HHP followed by MEF). MEF + HHP was performed by collecting the NaCl: biomass solution resulting from the last step of MEF per se (Fig. 1), centrifuging the mixture and collecting biomass to perform HHP extraction. After partitions of SM₂ resultant from both extraction procedures, the organic solvent containing the lipids was pooled for gravimetric determination of the lipids content. A similar procedure was used for HHP + MEF.

Table 2 presents the different conditions of parameters (electric field and/or pressure) applied in HHP and MEF combined assays. Individual HHP and MEF extractions were also performed to serve as controls. In order to allow comparisons, all extractions and/or exposures were conducted at 40 °C for 15 min, except for HHP, which was performed at 21 °C, according to the conclusions reached in HHP independent extractions.

Conditions used in combinations of HHP with MEF, performed with experimental conditions selected as the most promising, according to yields obtained in individual extractions (Table ​(Table11)

^*Exposure of biomass to NaCl solution