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2.2.4. Sample Analysis

KB Katelyn Brusach
SL Sarah Lorbach
JQ Jessica Quimby
EN Eline Nijveldt
RP Rene Paschall
HK Hannah Kinsella
VP Valerie Parker
RT Ramiro Toribio
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Radioimmunoassays (Human ghrelin (total) RIA; GHRT-89HK, and Human ghrelin (active) RIA; GHRA-88HK, Sigma-Aldrich) were used to measure AG and TG. Subsequently, DG was calculated from these values. All CKD and normal cat samples were analyzed in duplicate in the same assay run. Protocols were followed according to assay instructions (described above). Tubes were decanted using a timer to standardize the duration they were inverted and, then, were blotted to remove excess liquid from the lip of the tubes. Intra-assay coefficient of variation (using replicates from all samples type) was 4.1% for TG assay and 14.0% for AG assay.

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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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