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2.4. Quantification of Serum Amyloid A in Sera

SC Sophie Chagneau
MG Marie-Lou Gaucher
PF Philippe Fravalo
WT William P. Thériault
AT Alexandre Thibodeau
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Serum amyloid A (SAA) concentration in chicken sera was measured using a commercial ELISA kit: chicken serum amyloid A ELISA (Life diagnostics Inc., West Chester, PA, USA). According to the manufacturer’s instructions, ELISA was performed in duplicate for each standard and diluted serum sample. Absorbances were read at 450 nm using a Biochrom EZ Read 400 Microplate Reader (Biochrom, Cambridge, UK), and the concentrations were determined from the standard curve by a four-parameter logistic regression.

Copyright and License information:  ©2023 by the authors.
Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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