2.8.3. Reduced Glutathione Analysis

Glutathione (GSH) was extracted and identified during an untargeted metabolomics-based analysis. Due to its high relevance in mechanisms of oxidative stress, glutathione data are presented together with other oxidative stress biomarkers that were targeted. To obtain semi-quantitative GSH data, GC/MS-derived peak intensity values were standardised against the L-Alanine-d4 internal standard (i.e., [glutathione peak height/int. std. peak height] x int. std. concentration), assuming a similar response factor of 1.