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4.4. Effect of Fermentation Filtrate on M. enterolobii Egg Hatching

AS Aatika Sikandar
FG Fukun Gao
YM Yixue Mo
QC Qian Chen
RU Rana Muhammad Kaleem Ullah
HW Haiyan Wu
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About one hundred eggs, 200 µL of fermentation filtrate at varied concentrations (100%, 75%, 50%, 25%, 10%, and 5%), Czapek medium, and distilled water were poured into each 96-well plate well (He et al., 2020). The microliter plate was covered and put in a 28 °C incubator with constant humidity. An inverted microscope (ECLIPSE, Ti-S, Nikon) was used to observe the egg hatching at 2, 4, 6, and 8 days. Unhatched eggs were placed in distilled water to confirm A. tubingensis fermentation’s ovicidal or ovistatic properties [66]. In order to confirm the accuracy of the results, each treatment was carried out in triplicate. The cumulative hatching rate was determined by applying the following formula:

The hatching inhibition percentage was determined by using the following formula:

Copyright and License information:  ©2023 by the authors.
Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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